Goat Anti-Human IgG (H&L) FITC

  • Catalog number
    6915-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    Human IgG
  • Target Alternative Name
    Anti-human IgG, FITC-Linked Antibody, FITC linked secondary antibody
  • Antibody Type
    Secondary antibody
  • Species Reactivities
    Human
  • Immunogen Sequence
    Human IgG (H&L)
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
  • Purification
    Affinity Purified
  • Application
    IF, FACS
  • Application And Usages
    IF: 1:50 – 1:200, FC: 1:50 – 1:200.
  • Description
    Goat Polyclonal Secondary Antibody to Human IgG (H&L) FITC.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    6 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    Biovision supplies antibodies that are for research of human proteins.
  • Properties
    This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
    -
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    Goat Anti- IgG (H L) FITC
  • Technique
    anti-Human, anti-, anti, FITC, Goat, IgG, antibody to, antibodies, antibodies against human proteins, Fluorescein, goats, IgGs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    Human IgG, Heavy & Light chains, IgG
  • Label
    FITC
  • Species
    Human, Goats, Humans
  • Alternative name
    caprine Antibody toHuman Immunoglobulin G (heavy + light chain) fluorecein
  • Alternative technique
    antibodies, caprine, fluorescine, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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