Anti-FLAG Monoclonal Antibody

  • Catalog number
    GEN430079
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    FLAG
  • Other names
    FlaG; N/A; Uncharacterized flagellar protein FlaG; N/A; N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    flaG; N/A; N/A; N/A
  • Gene name synonims
    flaG; N/A; N/A; N/A
  • Other gene names
    flaG; N/A; N/A; N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG1/kappa
  • Clone
    N/A
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    Protein A & Ion exchange column
  • Form Appearance
    N/A
  • Concentration
    N/A
  • Storage and shipping
    Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.
  • Tested applications
    Western Blot, Immunocytochemistry, Immunoprecipitation, Flow Cyctometry, protein purification, protein localization, etc
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti- Monoclonal Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Label
    Flag
  • Alternative name
    Anti-Anti-FLAG Monoclonal Antibody
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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