Mouse Anti-Mouse Grp75 Monoclonal IgG1, Clone S52A-42
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Catalog numberSMC-133D-FITC
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PricePlease ask
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Size100 µg
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CloneS52A-42
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ImmunogenFusion protein amino acids 551-766 of mouse SALM2.
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Antibody s full descriptionMouse Anti-Mouse GRP75 Monoclonal IgG1 Antibody, Clone: S52A-42: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHSC74 Antibody, HSP74 Antibody, HSPA9 Antibody, HSPa9a Antibody, HSPA9B Antibody, Mortalin 2 Antibody, MOT2 Antibody, PBP74 Antibody
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Raised inMouse
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Antibody s targetGRP75
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Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking, Neuroscience, Neurodegeneration, Alzheimer's Disease, Parkinson's Disease
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, ELISA
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Antibody s reactivityHuman, Mouse, Rat, Nematode (Caenorhabditis elegans)
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Antibody s dilutionsWB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~75kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml was sufficient for detection of Grp75 in 10 µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
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Antibody in cellMitochondrion
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextGrp75, also known as mortalin, is a member of HSP70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with HSP60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (7). And finally, like its E.coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).
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Bibliography1. Kaul S.C., et al. (1993) Biochem Biophys Res Commun. 193: 348-355. 2. Wadhwa R., et al. (1993) J Biol Chem 268: 6615-6621. 3. Schneider H.C., et al. (1994) Nature 371: 768-774. 4. Manning-Krieg U.C., et al. (1991) EMBO J. 10: 3273-3280. 5. Wadhwa R., et al. (1998) J Biol Chem. 273: 29586-91. 6. Wadhwa R., et al. (2006) Int J Cancer 118: 2973-2980. 7. Kaul S.C., et al. (2003) Exp Cell Res. 286: 96-110. 8. Liberek K., et al. (1991) J Biol Chem. 266: 14491-14496. 9. Mizzen L.A., et al. (1991) Cell Regulation. 2: 165-179. 10. Leustek U.K., et al. (1989) PNAS USA. 86: 7805-7808.
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Released date7-Jan-2015
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PubMed number27183614, 24719326, 24719326, 23319326
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Tested applicationsWestern Blot, Western Blot, Immunocytochemistry/Immunofluorescence, ELISA
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Tested reactivityHuman, Human, Human, Human
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NCBI numberNP_004125.3
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Gene number3313
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Protein numberP38646
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 (SMC-133). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody (SMC-133) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondria. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Grp75 Antibody. (C) Composite. Western Blot analysis of Human HeLa cell lysates showing detection of Grp75 protein using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 (SMC-133). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody (SMC-133) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 (SMC-133). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody (SMC-133) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondria. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Grp75 Antibody. (C) Composite. Mouse Anti-Grp75 Antibody [S52A-42] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-133) Mouse Anti-Grp75 Antibody [S52A-42] used in Western Blot (WB) on Human HeLa cell lysates (SMC-133) Mouse Anti-Grp75 Antibody [S52A-42] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-133)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-42, IGLVI-42, IGHV3-42, IGKV1D-42, RNU6-42P, DSCAM, NFX1, KIR2DL1, OR5D2P
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Short nameMouse Anti-Mouse Grp75 Monoclonal IgG1, Clone S52A-42
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Grp75 monoclonal IgG1, clonality S52A-42
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Alternative techniquemurine, antibodies
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Clone nameS52A-42
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-42
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-42 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-42
- immunoglobulin lambda variable (I)-42 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-42 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-42
- immunoglobulin heavy variable 3-42 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-42 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1D-42
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 42, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 42
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameDS cell adhesion molecule
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Synonyms gene name
- Down syndrome cell adhesion molecule
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- I-set domain containing
- Fibronectin type III domain containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear transcription factor, X-box binding 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-05-26
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Pubmed identfication
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Classification
- PHD finger proteins
- Ring finger proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekiller cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1
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Synonyms gene name
- killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Killer cell immunoglobulin like receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 5 subfamily D member 2 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 5, subfamily D, member 11 pseudogene
- olfactory receptor, family 5, subfamily D, member 2 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-11-09
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 5
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
