Human Inosine-5’-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit

  • Catalog number
    K495-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    High-throughput compatible, quick and easy screening for Neprilysin inhibitors; Includes human Neprilysin.
  • Summary
    • Detection method- Colorimetric (OD 450 nm) • Application-Screening/characterizing/studying IMPDH inhibitors/activators
  • Detection Method
    Colorimetric (OD 450 nm)
  • Species Reactivity
    Human
  • Applications
    Screening/characterizing/studying IMPDH inhibitors/activators
  • Sample Type
    Not Applicable
  • Features Benefits
    • Simple and reliable test to screen IMPDH inhibitors/activators • High-throughput compatible • Includes Inhibitor Control (MPA)
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Inosine monophosphate dehydrogenase, IMPDH, (E.C. 1.1.1.205) is the rate-limiting enzyme in de novo guanine nucleotide biosynthesis and it is essential for lymphocyte proliferation. IMPDH oxidizes inosine 5’-monophosphate (IMP) to xanthine 5’-monophosphate (XMP) using NAD as a cofactor. It plays a critical role in cell growth and in the malignancy of some tumors such as pancreatic, colon and bladder cancers. IMPDH2 is the predominant isoform of IMPDH and is specifically linked to a wide range of cancers and lymphocyte proliferation. IMPDH is recognized as a validated target as antiviral, antiparasitic, antimicrobial, antileukemic and immunosuppressive agents. In BioVison’s IMPDH inhibitor screening assay, IMP is oxidized by IMPDH producing a series of intermediates, which react with the probe to generate a colorimetric signal (OD 450 nm). The signal is directly proportional to the IMPDH activity and has a very low background. In the presence of a IMPDH inhibitor such as mycophenolic acid (MPA), the reaction is arrested, thus decreasing the signal. BioVision’s IMPDH assay is fast, sensitive and reproducible and is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
  • Gene target
  • Short name
    Inosine-5’-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Species
    Humans
  • Alternative name
    H. sapiens Inosine-5’-Monophosphate Dehydrogenase (IMPDH) suppressor detection test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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