Protein A-Agarose

• Catalog number
  6526-5
• Price
  Please ask
• Size
  5 ml

• Description
  Reusable Protein A conjugated agarose beads with ≥20 mg/ml binding capacity & 2.89 ml/min flow rate for antibody purification from multiple sources. Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Protein A-Agarose is ≥ 20 mg human or rabbit IgG per ml of wet beads. Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
• Product Highlights
  •CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O. - HIGH BINDING CAPACITY: Binding of IgG ≥ 20 mg human or rabbit IgG/ml Protein A-Agarose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.89 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
• Storage Temp
  +4°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Properties
  The purest agarose was used in the production of Protein A-Agarose by Biovision.
• Conjugation
  Agarose

• Gene target
  Protein  
• Gene symbol
  CENPA, VAPA, AKAP10
• Short name
  Protein A-Agarose
• Technique
  Agarose, agaroses
• Label
  Agarose
• Alternative name
  Protein A-molecular sieve
• Alternative technique
  separation

Gene info

• Identity
  HGNC:1851
• Gene
  CENPA
• Long gene name
  centromere protein A
• Synonyms gene name
  • centromere protein A (17kD)
  • centromere protein A, 17kDa
• Synonyms
  • CENP-A
  • CenH3
• Synonyms name
  • centromere-specific histone
  • histone H3-like centromeric protein A
• GenBank acession
  • U14518
• Locus
  2p23.3
• Discovery year
  1988-05-31
• Entrez gene record
  1058
• RefSeq identity
  • NM_001809
• Classification
  • Constitutive centromere associated network
  • H3 histones
• VEGA ID
  OTTHUMG00000097073

Gene info

• Identity
  HGNC:12648
• Gene
  VAPA
• Long gene name
  VAMP associated protein A
• Synonyms gene name
  • VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
  • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• Synonyms
  • hVAP-33
  • VAP-A
• Locus
  18p11.22
• Discovery year
  1999-03-19
• Entrez gene record
  9218
• Pubmed identfication
  • 9920726
  • 9657962
• VEGA ID
  OTTHUMG00000131603

Gene info

• Identity
  HGNC:368
• Gene
  AKAP10
• Long gene name
  A-kinase anchoring protein 10
• Synonyms gene name
  • A kinase (PRKA) anchor protein 10
• Synonyms
  • D-AKAP2
  • PRKA10
  • MGC9414
• Synonyms name
  • dual-specificity A-kinase anchoring protein 2
  • protein kinase A anchoring protein 10
  • mitochondrial A kinase PPKA anchor protein 10
• GenBank acession
  • AF037439
• Locus
  17p11.2
• Discovery year
  1999-09-16
• Entrez gene record
  11216
• Pubmed identfication
  • 9326583
• RefSeq identity
  • NM_007202
• Classification
  • A-kinase anchoring proteins
• VEGA ID
  OTTHUMG00000059515

MeSH Data

• Name
  Electrophoresis, Agar Gel
• Concept
  Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
• Tree numbers
  • E05.196.401.153
  • E05.301.300.100
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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