Description
Reusable Protein A conjugated agarose beads with ≥20 mg/ml binding capacity & 2.89 ml/min flow rate for antibody purification from multiple sources. Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Protein A-Agarose is ≥ 20 mg human or rabbit IgG per ml of wet beads. Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
Product Highlights
•CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O. - HIGH BINDING CAPACITY: Binding of IgG ≥ 20 mg human or rabbit IgG/ml Protein A-Agarose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.89 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Storage Temp.
+4°C
Shipping
gel pack
Shelf Life
12 months
Notes
For research use only. Not to be used for human or animal treatment or consumption.
Properties
The purest agarose was used in the production of Protein A-Agarose by Biovision.
Conjugation
Agarose
Gene targetProtein
Short name
Protein A-Agarose
Technique
Agarose
Label
Agarose
Alternative name
Protein A-molecular sieve
Alternative technique
separation
