Recombinant Human NBL1 / DAND1 / DAN Protein (His tag)

• Catalog number
  PKSH031808-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  D1S1733E;DAN;DAND1;NB;NO3
• Activity
  Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.
• Sequence
  Met 1-Asp 180
• Fusion tag
  C-His
• Accession
  NP_005371.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞98 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  19.3 kDa
• AP Mol Mass
  27 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NBL1   DAND1   DAN   Protein   His   tag  
• Gene symbol
  NBL1, MICOS10-NBL1, PARN
• Short name
  Recombinant NBL1 / DAND1 / DAN Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens NBL1 / DAND1 / DAN Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7650
• Gene
  NBL1
• Long gene name
  NBL1, DAN family BMP antagonist
• Synonyms gene name
  • neuroblastoma, suppression of tumorigenicity 1
  • neuroblastoma 1, DAN family BMP antagonist
• Synonyms
  • D1S1733E
  • NB
  • DAN
  • NO3
  • DAND1
• Synonyms name
  • neuroblastoma candidate region, suppression of tumorigenicity 1
  • neuroblastoma suppressor of tumorigenicity 1
  • differential screening-selected gene aberrant in neuroblastoma
• Locus
  1p36.13
• Discovery year
  1997-11-20
• Entrez gene record
  4681
• Pubmed identfication
  • 7633401
  • 24810382
• RefSeq identity
  • NM_005380
• Classification
  • DAN family
• VEGA ID
  OTTHUMG00000002700

Gene info

• Identity
  HGNC:48338
• Gene
  MICOS10-NBL1
• Long gene name
  MICOS10-NBL1 readthrough
• Synonyms gene
  • MINOS1-NBL1
• Synonyms gene name
  • MINOS1-NBL1 readthrough
• Locus
  1p36.13
• Discovery year
  2013-05-09
• Entrez gene record
  100532736
• VEGA ID
  OTTHUMG00000183956

Gene info

• Identity
  HGNC:8609
• Gene
  PARN
• Long gene name
  poly(A)-specific ribonuclease
• Synonyms gene name
  • poly(A)-specific ribonuclease (deadenylation nuclease)
• Synonyms
  • DAN
• Synonyms name
  • deadenylation nuclease
• GenBank acession
  • AJ005698
• Locus
  16p13.12
• Discovery year
  1998-07-23
• Entrez gene record
  5073
• Pubmed identfication
  • 10640832
• RefSeq identity
  • NM_002582
• Classification
  • Ribosomal biogenesis factors
  • PARN exonuclease family
• VEGA ID
  OTTHUMG00000173199
• Locus Specific Databases
  • LRG_1286

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products