PCNA Antibody, ALEXA FLUOR 488

• Catalog number
  bs-0754R-A488
• Price
  Please ask
• Size
  100ul

• Long name
  PCNA Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
• Also known as
  Anti-PCNA PAb ALEXA FLUOR 488
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 488
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  PCNA
• Specificity
  This is a highly specific antibody against PCNA.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  220-260/261
• Subcellular location
  Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human PCNA
• Gene ID Number
  5111
• Swiss Prot
  P12004
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  499nm/519nm
• Synonyms
  ATLD2; Proliferating cell nuclear antigen; PCNA; Cyclin
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-PCNA has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor
• French translation
  anticorps

• Gene target
  PCNA  
• Gene symbol
  PCNA, PCNA-AS1
• Short name
  Anti-PCNA
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 488
• Alternative name
  PCNA Polyclonal Antibody, ALEXA FLUOR 488
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:8729
• Gene
  PCNA
• Long gene name
  proliferating cell nuclear antigen
• GenBank acession
  • J04718
• Locus
  20p12.3
• Discovery year
  1990-01-15
• Entrez gene record
  5111
• Pubmed identfication
  • 2565339
• VEGA ID
  OTTHUMG00000031798

Gene info

• Identity
  HGNC:37184
• Gene
  PCNA-AS1
• Long gene name
  PCNA antisense RNA 1
• Synonyms gene
  • PCNAAS
  • PCNA-AS
• Synonyms gene name
  • PCNA antisense RNA (non-protein coding)
  • PCNA antisense RNA 1 (non-protein coding)
• Locus
  20p12.3
• Discovery year
  2009-08-18
• Entrez gene record
  100302739
• Pubmed identfication
  • 10488129
  • 24704293
• RefSeq identity
  • NR_028370
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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