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Synonyms
Ubiquitin-Aminomethylcoumarin
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Alternative_names
Ubiquitin-Aminomethylcoumarin
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Description
An industry standard substrate for deubiquitinylating enzymes (DUBs)
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Recombinant
Yes
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Purity
≥95%
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Molecular Weight
8.6 Kda
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
1 mg/ml (DMSO)
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Appearance
Liquid
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Physical form description
A solution in DMSO ( 1 mg/ml)
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Background Information
Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039uM) and for Isopeptidase-T (Km = 0.17-1.4uM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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