Prothrombin Antibody

• Catalog number
  A1114-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  F2
• Target Alternative Name
  Coagulation factor II
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Human Prothrombin (Cat. No. 7684-1)
• Accession
  P00734
• Gene Id
  2147
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  In PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol
• Purification
  Affinity purified rabbit IgG
• Application
  WB
• Positive Control
  WB: h Prothrombin, h serum
• Application And Usages
  WB: 1-4 µg
• Description
  Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Prothrombin  
• Gene symbol
  F7
• Short name
  Prothrombin Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Prothrombin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3544
• Gene
  F7
• Long gene name
  coagulation factor VII
• Synonyms gene name
  • coagulation factor VII (serum prothrombin conversion accelerator)
• Synonyms name
  • eptacog alfa
  • FVII coagulation protein
  • factor VII
• Locus
  13q34
• Discovery year
  2001-06-22
• Entrez gene record
  2155
• Pubmed identfication
  • 3264725
  • 2511201
• RefSeq identity
  • NM_000131
• Classification
  • Gla domain containing
• VEGA ID
  OTTHUMG00000017373
• Locus Specific Databases
  • LRG_554

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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