FITC-conjugated Goat Anti-HNE (Hydroxynonenal)
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Catalog number
MBS390042
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Price
Please ask
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Size
0.1 mL
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Products_type
Antibody
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Products_short_name
[HNE (Hydroxynonenal)]
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Products_name_syn
[FITC conjugated Goat Anti- Hydroxynonenal (HNE), Polyclonal Antibody]
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Products_gene_name
[HNE]
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Specificity
Specifically binds to HNE modified protein. Molar F/P ratio is 4.7. The Investigator should determine working dilutions.
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Form
0.2 ml in Freeze-dried powder
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Concentration
1 mg / ml
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Storage_stability
Freeze-dried product should be refrigerated until opened. After opening, restore to 0.2 ml with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4 degree C as an undiluted liquid. Do not use for more than one day after dilution. The products are light sensitive and should be keep in dark. To avoid repeated freezing and thawing after reconstitution, we suggest aliquots and storage at -20 degree C.
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Tested_application
Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Gene symbol
ELANE
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Short name
FITC-conjugated Goat Anti-HNE (Hydroxynonenal)
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Technique
anti-, anti, FITC, Goat, antibody to, antibodies, antibody Conjugates, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
FITC
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Species
Goats
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Alternative name
fluorecein-coupled caprine Antibody toHNE (Hydroxynonenal)
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Alternative technique
antibodies, caprine, fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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