mPGES-1 glutathione dependent prostaglandin E synthase protein, positive control

• Catalog number
  AS03 031S
• Price
  Please ask
• Size
  50 µl (20.5 µg)

• Available ordering format
  Liquid
• Immunogen
  to be determined
• Raised in
  N/A
• Clonality
  N/A
• Clone
  N/A
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB), immunolocalization (blocking protein for antibody)
• Connected products
  AS03 031 | mPGES-1 glutathione dependent prostaglandin E synthase antibodies
• Recommended dilutions for use
  positive control: 5 μl load per well is optimal for most chemiluminescent detection systems.preabsorption: 6-12 ug of protein/100ul diluted anti-mPGES-1 serum (1:5000)
• Molecular weight expected аpparent
  16-17 kDa
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  no confirmed exceptions from predicted reactivity currently known
• Supplementary information
  Concentration: 0.41 µg/µl.Western blot: recommended load per gel to allow Coomasie staining and western blot detection is 5 µlImmunolocalization: 6-12 ug of protein/100 µl diluted anti-mPGES-1 serum (1:5000) was used for preabsorption of mPGES-1 antibodies followed by immunolocalization.
• References
  to be added when available
• Scientific context
  mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.
• Notes
  Protein is provided in 10 mM NaPI, pH 8, 150 mM KCl, 0.1 mM EDTA, 1mM GSH, 10 % glycerol, 0,1 % reduced Triton X-100. It has been produced in E.coli.If intended to be used in Western blot, loading buffer should be added and the solution should be heated up in 98°C for 5 minutes before loading on the gel.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
• Group
  positif

• Gene target
  mPGES-1   glutathione   dependent   prostaglandin   synthase   protein  
• Short name
  mPGES-1 glutathione dependent prostaglandin E synthase protein, positive control
• Technique
  Positive, Control, controls
• Alternative name
  mPGES-1 GSH dependent prostaglandin E synthase protein, positive reference
• Alternative technique
  controls
• Tissue
  control

Similar products