Recombinant Human Pyruvate Kinase, Liver And RBC/PKLR Protein(C-6His)

• Catalog number
  PKSH032984-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  Pyruvate Kinase Isozymes R/L; Pyruvate Kinase 1; R-Type/L-Type Pyruvate Kinase; Red Cell/Liver Pyruvate Kinase; PKLR; PK1; PKL
• Activity
  NA
• Sequence
  Met1-Ser574
• Fusion tag
  C-6His
• Accession
  P30613
• Expressed Host
  Human cells
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞95% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
• Mol Mass
  62.9 kDa
• AP Mol Mass
  58 kDa
• Formulation
  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT 0.2M NaCl, 10% glycerol, pH 8.0.
• Background
  Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Pyruvate   Kinase   Liver   RBC/PKLR   Protein   C-6His  
• Gene symbol
  PKLR
• Short name
  Recombinant Pyruvate Kinase, Liver RBC/PKLR Protein(C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Pyruvate phosphorylation catalyst, Liver And RBC/PKLR Protein(C-6His)
• Alternative technique
  rec
• Tissue
  liver

Gene info

• Identity
  HGNC:9020
• Gene
  PKLR
• Long gene name
  pyruvate kinase L/R
• Synonyms gene name
  • pyruvate kinase, liver and RBC
• GenBank acession
  • BC025737
  • M15465
• Locus
  1q22
• Discovery year
  2001-06-22
• Entrez gene record
  5313
• Pubmed identfication
  • 3566732
• RefSeq identity
  • NM_000298
• VEGA ID
  OTTHUMG00000035875
• Locus Specific Databases
  • LRG_1136

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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