GADD153 Antibody

• Catalog number
  3421-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  GADD 153
• Target Alternative Name
  GADD153 , DDIT3 , 126337 ,P35638 , CEBPZ , CHOP , CHOP10 , GADD153 , mgC4154
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 124 of human GADD153
• Accession
  P35638
• Gene Id
  1649
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.2 mg/ml
• Formulation
  100 µg (0.2 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blotting, Immunoprecipitation, Immunohistochemistry
• Application And Usages
  Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 25 kDa GADD153 of human, mouse, and rat origins.
• Description
  GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GADD153  
• Gene symbol
  DDIT3
• Short name
  GADD153 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  GADD153 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2726
• Gene
  DDIT3
• Long gene name
  DNA damage inducible transcript 3
• Synonyms gene name
  • DNA-damage-inducible transcript 3
• Synonyms
  • CHOP10
  • GADD153
  • CHOP
• Synonyms name
  • C/EBP zeta
• GenBank acession
  • BC003637
• Locus
  12q13.3
• Discovery year
  1991-09-11
• Entrez gene record
  1649
• Pubmed identfication
  • 1990262
• RefSeq identity
  • NM_004083
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000170046

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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