This antibody has been succesfully tested for use in following methods: Western blotting, Immunoprecipitation, Immunohistochemistry
Target of this antibody: GADD 153
Formulation of this antibody: 100 µg (0.2 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target: GADD153 , DDIT3 , 126337 ,P35638 , CEBPZ , CHOP , CHOP10 , GADD153 , MGC4154
Recommended usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 25 kDa GADD153 of human, mouse, and rat origins.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide surrounding amino acid 124 of human GADD153
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
Form of antibody: Liquid
Concentration of antibody: 0.2 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps