anti- Aconitase 1 antibody

• Catalog number
  FNab00087
• Price
  Please ask
• Size
  2X100μg

• Form
  liquid
• Purification
  Immunogen affinity purified
• Purity
  ≥95% as determined by SDS-PAGE
• Storage
  PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
• Background
  The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
• Immunogen
  aconitase 1, soluble
• Synonyms
  ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1
• Calculated MW
  120 kDa
• Uniprot ID
  P21399
• Specificity
  Human, Mouse, Rat
• Tested Application
  ELISA, WB, IHC
• Recommended dilution
  WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
• Image1
  Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab00087(ACO1 antibody) at dilution of 1:100
• Image4
  Hela cells were subjected to SDS PAGE followed by western blot with FNab00087(ACO1 antibody) at dilution of 1:1000
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Aconitase  
• Gene symbol
  ACO1, IBA57
• Short name
  anti- Aconitase 1 antibody
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit
• Isotype
  IgG
• Alternative name
  antibody to- Aconitase 1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:117
• Gene
  ACO1
• Long gene name
  aconitase 1
• Synonyms gene
  • IREB1
• Synonyms gene name
  • aconitase 1, soluble
• Synonyms
  • IRP1
  • IREBP
• Synonyms name
  • aconitate hydratase, cytoplasmic
  • soluble aconitase
  • cytosplasmic aconitase
• GenBank acession
  • M58510
• Locus
  9p21.1
• Discovery year
  1989-01-01
• Entrez gene record
  48
• Pubmed identfication
  • 2172968
  • 2771641
• RefSeq identity
  • NM_002197
• VEGA ID
  OTTHUMG00000019740

Gene info

• Identity
  HGNC:27302
• Gene
  IBA57
• Long gene name
  iron-sulfur cluster assembly factor IBA57
• Synonyms gene
  • C1orf69
• Synonyms gene name
  • chromosome 1 open reading frame 69
  • IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae)
  • IBA57 homolog, iron-sulfur cluster assembly
• Synonyms
  • FLJ12734
• Synonyms name
  • iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins, with a mass of 57kDa
• GenBank acession
  • AK022796
• Locus
  1q42.13
• Discovery year
  2005-05-18
• Entrez gene record
  200205
• Pubmed identfication
  • 22323289
• RefSeq identity
  • NM_001010867
• Classification
  • Mitochondrial iron-sulfur assembly components
• VEGA ID
  OTTHUMG00000039769

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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