Human CellExp™ ErbB1/EGFR, mouse recombinant
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Catalog number7402-10
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PricePlease ask
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Size10 ug
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SynonymsEGFR, ERBB, ERBB1, HER1, PIG61, mENA
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Alternative_namesEGFR, ERBB, ERBB1, HER1, PIG61, mENA
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DescriptionA cell-surface receptor for members of the EGF-family of extracellular protein ligands.
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 ug/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 ug/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
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Molecular WeightThis protein has a C-terminal 6×His tag and has a calculated MW of 70 kDa. The predicted N-terminus is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationThe epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolEGFR-AS1, EGILA, EGFR, RHBDF1
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Short nameCellExp™ ErbB1/EGFR, mouse recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesHumans, Mouses
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Alternative nameH. sapiens CellExp™ ErbB1/epidermal growth factor receptor, mouse Rec.
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Alternative techniquerec, murine
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Alternative to gene targetepidermal growth factor receptor, ERBB and ERBB1 and HER1 and mENA and PIG61, EGFR and IDBG-16933 and ENSG00000146648 and 1956, transferase activity, nuclei, Egfr and IDBG-151194 and ENSMUSG00000020122 and 13649, EGFR and IDBG-644035 and ENSBTAG00000011628 and 407217
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Gene info
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Identity
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Gene
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Long gene nameEGFR antisense RNA 1
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Locus
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Discovery year2012-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEGFR interacting lncRNA
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Synonyms
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Locus
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Discovery year2019-09-27
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Pubmed identfication
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Classification
- Long non-coding RNAs with non-systematic symbols
Gene info
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Identity
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Gene
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Long gene nameepidermal growth factor receptor
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Synonyms gene
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Synonyms gene name
- epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Erb-b2 receptor tyrosine kinases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namerhomboid 5 homolog 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 8
- rhomboid family 1 (Drosophila)
- rhomboid 5 homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Rhomboid family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
