Alternative names of antibody target:
His tag, HHHHHH tag
A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Clonality of antibody:
This antibody reacts with:
6xHis synthetic peptide
Concentration of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation/IC
Western blotting (1 µg/ml), immunoprecipitation (40 µg/ml), and immunocytochemistry (2 µg/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.