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Anti-CREB-1(Ser121) (Polyclonal), ALEXA Fluor 594

Anti-CREB-1(Ser121) (Polyclonal), ALEXA Fluor 594 is available 1 time from Bioss polyclonal antibodies labs

GENTObs-5270R-A594 | Anti-CREB-1(Ser121) (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 600.11 USD

Catalog number GENTObs-5270R-A594
Price 600.11 USD
Size 100 microliters
1. Gene info
Identity 18855
Gene CREB3L3
Long gene name cAMP responsive element binding protein 3 like 3
Synonyms
  • CREB-H
  • CREBH
Synonyms name
  • cAMP-responsive element-binding protein, hepatic-specific
Locus 19p13.3
Discovery year 2003-12-09
Entrez gene record 84699
Pubmed identfication
  • 11353085
RefSeq identity
  • NM_032607
Classification
  • CREB3 transcription factor family
Havana BLAST/BLAT OTTHUMG00000181846
2. Gene info
Identity 2345
Gene CREB1
Long gene name cAMP responsive element binding protein 1
GenBank acession
  • M27691
Locus 2q33.3
Discovery year 1990-02-05
Entrez gene record 1385
RefSeq identity
  • NM_134442
Classification
  • Basic leucine zipper proteins
Havana BLAST/BLAT OTTHUMG00000132936
Product images
    Hela cells were fixed with 70% ice-cold methanol overnight at 4??permeabilized with 90% ice-cold methanol for 20 min at -20? and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CREB-1(Ser121) Polyclona
Type Conjugated Primary Antibody
Conjugated with ALEXA FLUOR® 594
Host organism Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide CREB-1Ser121
Specificity This antibody reacts specifically with CREB-1(Ser121)
Modification Phosphorylation
Modification site Ser121
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Subcellular locations N/A
Antigen Source KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser121 [ TD(p-S)QK]
Gene ID 1385
Swiss Prot N/A
Applications IF(IHC-P)
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission 590nm/617nm
Synonyms CREBSer121; p-CREB-1Ser121; pCREB-1Ser121; Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN.
Also known as CREB-1(Ser121) Polyclonal Antibody
Other name Anti-CREB-1(Ser121) Polyclonal
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene targetCREB-1(Ser121
Short name Anti-CREB-1(Ser121)
Technique Polyclonal, anti
Isotype Immunoglobulin G (IgG)
Label ALEXA FLUOR 594
Alternative name CREB-1(Ser121) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Alternative technique polyclonals
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Anti-CREB-1(Ser121) | Technique alternative | 01011212295
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