PicoProbe™ Reduced Glutathione (GSH) Assay Kit (Fluorometric)

• Catalog number
  K740-100
• Price
  Please ask
• Size
  100 assays

• Description
  It specifically detects reduced glutathione. Oxidized GSSG is not detected.
• Summary
  • Detection method- Fluorescence (535/587 nm) • Species reactivity- Mammalian • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
• Detection Method
  Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species Reactivity
  Mammalian
• Applications
  The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple procedure; takes only ~1-2 hours • Fast and convenient • Specific: does not detect oxidized GSSG • Sensitive: detects as low as 8 pmol GSH
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glutathione (GSH), a thiol-containing tripeptide, is a key antioxidant in living systems. Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell. BioVision’s PicoProbeTM Reduced Glutathione Assay Kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). Our assay is the most specific and sensitive in the market. Oxidized Glutathione does not interfere with the assay. The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  Reduced   Glutathione   GSH   Kit   Fluorometric  
• Gene symbol
  GSX1
• Short name
  ™ Reduced Glutathione (GSH) Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ Reduced GSH (GSH) test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:20374
• Gene
  GSX1
• Long gene name
  GS homeobox 1
• Synonyms gene
  • GSH1
• Synonyms
  • Gsh-1
• GenBank acession
  • AB044157
• Locus
  13q12.2
• Discovery year
  2003-02-18
• Entrez gene record
  219409
• RefSeq identity
  • NM_145657
• Classification
  • HOXL subclass homeoboxes
• VEGA ID
  OTTHUMG00000016637

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