PDK1 Antibody (pSer241): FITC

• Catalog number
  SPC-1050D-FITC
• Price
  Please ask
• Size
  100 µl

• Alernative name
  Rabbit Anti-Human PDK1 (pSer241) Polyclonal
• Target
  PDK1 (pSer241)
• Conjugate
  FITC
• Research Area s
  Cell Signaling , Cytoskeleton , Microfilaments , Actin , Cell Signaling , Protein Phosphorylation , Serine / Threonine Kinases
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  A phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1 (AA238-244)
• Immunogen Species
  Human
• Gene ID
  5170
• Swiss Prot
  O15530
• Applications
  WB , AM
• Host Species
  Rabbit
• Species Reactivity Full Name
  Human
• Storage Buffer
  PBS pH7.4, 50% glycerol, 0.025% Thimerosal
• Concentration
  NA
• Specificity
  Detects ~63kDa.
• Storage Temperature
  -20°C
• Shipping Temperature
  Blue Ice or 4°C
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  PDK1   pSer241  
• Gene symbol
  PDK1, PDPK1
• Short name
  PDK1 Antibody (pSer241): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  PDK1 (antibody to-) (pSer241): fluorecein
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:8809
• Gene
  PDK1
• Long gene name
  pyruvate dehydrogenase kinase 1
• Synonyms gene name
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1
• GenBank acession
  • L42450
• Locus
  2q31.1
• Discovery year
  1996-08-14
• Entrez gene record
  5163
• Pubmed identfication
  • 7499431
• RefSeq identity
  • NM_002610
• VEGA ID
  OTTHUMG00000132285

Gene info

• Identity
  HGNC:8816
• Gene
  PDPK1
• Long gene name
  3-phosphoinositide dependent protein kinase 1
• Synonyms
  • PDK1
• Synonyms name
  • PkB kinase
• GenBank acession
  • AF017995
• Locus
  16p13.3
• Discovery year
  1998-03-23
• Entrez gene record
  5170
• Pubmed identfication
  • 9094314
  • 9445477
• Classification
  • AGC family kinases
• VEGA ID
  OTTHUMG00000128874

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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