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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser296 of human RAF1 (AA293-299)
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Antibody s target
Human RAF1 (pSer296)
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Antibody s full description
Rabbit Anti-Human RAF1 (pSer296) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
c Raf Antibody, C-raf Antibody, C-Raf proto-oncogene, serine/threonine kinase Antibody, CMD1NN Antibody, Craf 1 transforming gene Antibody, cRaf Antibody, Craf1 transforming gene Antibody, EC 2.7.11.1 Antibody, kinase Raf1 Antibody, Murine sarcoma 3611 oncogene 1 Antibody, NS5 Antibody, Oncogene MIL Antibody, Oncogene RAF1 Antibody, OTTHUMP00000160218 Antibody, OTTHUMP00000207813 Antibody, OTTHUMP00000209389 Antibody, Protein kinase raf 1 Antibody, Proto-oncogene c-RAF Antibody, Raf 1 Antibody, Raf 1 proto oncogene serine/threonine kinase Antibody, RAF Antibody, Raf proto oncogene serine/threonine protein kinase Antibody, RAF proto-oncogene serine/threonine-protein kinase Antibody, RAF-1 Antibody, RAF1 Antibody, RAF1_HUMAN Antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 Antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV Antibody, v raf 1 murine leukemia viral oncogene homolog 1 Antibody, v-raf murine sarcoma viral oncogene homolog 1 Antibody, vraf1 murine leukemia viral oncogene homolog 1 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~73kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1071 was sufficient for detection of RAF1 (pSer296) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cell Membrane, Mitochondrion, Nucleus
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Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2
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Scientific context
RAF1 is a protein-serine/threonine kinase. RAF-1 is upregulated in many cancers since Ras (an upstream activator of RAF) is mutated/upreguated in many cancers. For example, RAF-1 is over-expressed due to oncogenic ras mutations in about 35% of Non-Small Cell Lung Cancer (NSCLC). A small number of RAF mutations have been found in stomach and mouth tumours. RAF-1 gene amplification was detected in 4% of bladder cancer samples, while deletions at the RAF-1 locus were detected in 2.2% of these samples; these amplifications and deletions were correlated with tumour grade.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002871
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Gene number
5894
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Protein number
P04049
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RNASE3, RAF1
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Short name
Rabbit Anti- RAF1 (pSer296) Polyclonal: ATTO 700
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 700
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman v-raf-1 murine leukemia viral oncogene homolog 1 (pSer296) polyclonal: ATTO 700
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
v-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196
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