This antibody has been succesfully tested for use in following methods:
Western blot, ELISA, ChIP, Dot blot.
Target of this antibody:
Formulation of this antibody:
In PBS with 0.05% (W/V) sodium azide.
Alternative names of antibody target:
WB: 1:250, ELISA: 1:500, Dot Blot: 1:20,000, ChIP: 5 µl/ChIP.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Whole antiserum from rabbit
KLH-conjugated synthetic peptide of Histone H4 containing the acetylated lysine 8
How to handle this antibody:
The antibody solution should be gently mixed before use.
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active gene transcription.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.