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Immunogen
Full-length GFP protein.
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Antibody s target
GFP
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Antibody s full description
Mouse Anti-GFP Polyclonal
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Primary research fields
Tags and Cell Markers, Fluorescent Proteins
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
GFP Antibody, Green fluorescent protein Antibody, yfp Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Mouse
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Antibody s reactivity
Species Independent
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Antibody s recommended dilutions for use
WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Recognizes GFP, EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:10000 dilution of SPC-1250 was sufficient for detection of GFP in 10 µg of GFP Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Contact our support service.
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Tissue specificity
Photocytes.
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Scientific context
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, and is excited by and brightly fluoresces green (emission peak at a wavelength of 509nm) when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
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Bibliography
1. Prendergast F.G. and Mann K.G. (1978) Biochemistry. 17 (17): 3448–53. 2. Tsien R.Y. (1998) Annual Review of Biochemistry. 67: 509–44.
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Released date
6/Sep/2016
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Aequorea victoria GFP Protein showing detection of ~26.9kDa GFP protein using Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Predicted/Observed Size: ~26.9kDa. Mouse Anti-GFP Antibody used in Western blot (WB) on GFP Protein (SPC-1250)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
