anti-MIP-1a Antibody

  • Catalog number
    XP-5233
  • Price
    Please ask
  • Size
    0.1 mg
  • Gene info
    Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
  • Gene names
    Scya3, MIP-1a, Mip1a, Scya3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha
  • Antibodie s label
    unlabelled serum
  • Purity
    Anti-Rat MIP-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat MIP-1alpha matrix.
  • Ab raised in
    Rabbit
  • Type
    Polyclonal
  • Antigen
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MIP-1alpha (Macrophage Inflammatory Protein -1 alpha).
  • Immuno application
    ELISA, Western Blot, NELISAut
  • Antibody advice
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP-1a; (100 ng/mL), a concentration of 8.0 - 10.0 ug/mL of this antibody is required. ELISA:To detect Rat MIP-1a; by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MIP-1a;. Sandwich:To detect Rat MIP-1a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MIP-1a; (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1a;.Western Blot:To detect Rat MIP-1a; by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1a; is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Keep at
    MIP-1a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
  • Works with
    Rat
  • Omim nr
    1709024
  • Ncbi nr
    P50229
  • Vial with antibody
    Lyophilized
  • NCBI GENE nr
    25542
  • GENE symbol
    Ccl3
  • Antigene name
    chemokine (C-C motif) ligand 3
  • Source
    Rattus norvegicustus norvegicus
  • Protein nr
    P50229
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    MIP-1a  
  • Gene symbol
    MIPEP, TNPO1, MIP, CCL26, TRAPPC2B, CCL15, TRAF3IP1, MAFIP, TRAPPC2, CCL23
  • Short name
    anti-MIP-1a Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative name
    Antibody toMIP-1a (antibody to-)
  • Alternative technique
    antibodies
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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