Midkine, murine recombinant

• Catalog number
  7158-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  MK, NEGF-2
• Alternative_names
  MK, NEGF-2
• Description
  Plays an important regulatory role in epithelial-mesenchymal interactions
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml.
• Biological activity
  Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml.
• Molecular Weight
  13.3 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 0.4 x PBS, pH 7.4.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorigenesis. Recombinant murine Midkine is a 13.3 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds.
• Amino acid sequence
  VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Midkine   murine  
• Short name
  Midkine, murine recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Midkine, murine Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Urine Specimen Collection
• Concept
  Scope note: Methods or procedures used to obtain samples of URINE.
• Tree numbers
  • E01.370.225.998.762
  • E05.200.998.762
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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