PCNA Antibody
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Catalog number3350R-100
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PricePlease ask
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Size100 µg
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Antibody TargetPCNA
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Target Alternative NamePCNA, proliferating cell nuclear antigen
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide corresponding to the C-terminus of human PCNA
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AccessionCAG38740.1
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Gene Id5111
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation100 µg (0.2 mg/ml) affinity purified rabbit anti-PCNA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml). Per researcher’s feedback, it can also be used in immunoprecipitation and Immunohistochemistry (frozen & paraffin). However, the optimal conditions should be determined individually.
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DescriptionPCNA (Proliferating Cell Nuclear Antigen) is a 36 kDa protein also known as cyclin. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. There are two forms of PCNA protein present in cells, a soluble form sensitive to organic fixation and not involved in replication, and the other form is insoluble and is associated with ongoing DNA synthesis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPCNA, PCNA-AS1
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Short namePCNA Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namePCNA (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameproliferating cell nuclear antigen
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GenBank acession
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Locus
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Discovery year1990-01-15
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePCNA antisense RNA 1
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Synonyms gene
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Synonyms gene name
- PCNA antisense RNA (non-protein coding)
- PCNA antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2009-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data