HAT Activity Fluorometric Assay Kit

  • Catalog number
    K334-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Sensitive assay to measure HAT activity
  • Summary
    • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications- - Measurement of HAT activity in Nuclear Extracts - Measurement of HAT activity of purified enzyme preparations
  • Detection Method
    Fluorescence (Ex/Em = 535/587 nm)
  • Applications
    • Measurement of HAT activity in Nuclear Extracts • Measurement of HAT activity of purified enzyme preparations
  • Sample Type
    • Nuclear extracts from cells and tissue • Recombinant enzyme
  • Features Benefits
    • Simple, rapid & convenient • The assay can detect HAT Activity less than 6 mU in a variety of samples
  • Storage Conditions
    -80°C
  • Shipping Conditions
    dry ice
  • Shelf life
    12 months
  • Background
    Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
  • Gene symbol
    TMPRSS11D
  • Short name
    HAT Activity Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    HAT Activity fluorescence test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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