This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Alternative names of antibody target:
RPS6KA3 , CLS , RSK-2 , RSK2 , p90-RSK2 , ISPK1 , MRX19 , HU-3 , pp90RSK2 , MAPKAPK1B , HU-2 , RSK , S6K-alpha3 , ISPK-1
Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~94 kDa RSK2 of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
E. coli expressed recombinant human RSK2
How to handle this antibody:
The antibody solution should be gently mixed before use.
RSKs (90 kDa family of ribosomal S6 kinases), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the RSK family is that each possesses two non-identical complete kinase catalytic domains.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.