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Category
Primary Antibodies
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Long description
Used as a marker for human myeloid cells. p13 protein is a nuclear antigen expressed in human granulocytes and monocytes in lymphoid and non-lymphoid tissues and is expressed during the early phases of myeloid differentiation.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with nuclei from Pokeweed mitogen stimulated with peripheral blood lymphocytes and mouse myeloma cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
BM-3
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Antigen antibody binding interaction
Mouse anti p13 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry (Frozen Sections, Not Paraffin), Flow Cytometry
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Antibody s suited for
Antibody can be used for immunohistochemistry on frozen tissues sections and flow cytometry. Not for use on paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Epstein, A.L. and Clevenger, C.V. 'Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immunobiochemical methods using monoclonal antibodies.' Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp. 117-137_x000B__x000B_2. Murao, S., et al. 'Expression of maturation-specific nuclear antigens in differentiating human myeloid leukemia cells.' Cancer Research 1985: 45:791-795
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
