EGFP, Enhanced Green Fluorescence Protein

• Catalog number
  AS13 2700
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  Recombinant EGFP, UniProt: 31711420
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution please add 100 µl of sterile water
• Storage condition
  After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
• Verified applications
  Immunohistochemistry (IHC), Western blot (WB)
• Connected products
  no related to other products
• Recommended dilutions for use
  1:1000-1: 5000 (IHC and WB)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  EGFP
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  EGFP has Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000.Optimal dilutions/concentrations of this antibody should be determined by the end user.
• References
  to be added when available
• Scientific context
  EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Conjugate
  The Green Fluorescent Protein (eGFP) of , Enhanced Protein can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
• Test
  Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  Enhanced   Protein  
• Gene symbol
  LY6G6F-LY6G6D, ERI3, PPP1R14C
• Short name
  , Enhanced Protein
• Label
  green
• Alternative name
  EGFP, Enhanced Green Fluorescence Protein

Gene info

• Identity
  HGNC:38821
• Gene
  LY6G6F-LY6G6D
• Long gene name
  LY6G6F-LY6G6D readthrough
• Synonyms
  • MEGT1
  • MEGT-1
  • G6F-LY6G6D
• Synonyms name
  • megakaryocyte-enhanced gene transcript 1 protein
• Locus
  6p21.33
• Discovery year
  2019-10-23
• Entrez gene record
  110599563
• Pubmed identfication
  • 18817541

Gene info

• Identity
  HGNC:17276
• Gene
  ERI3
• Long gene name
  ERI1 exoribonuclease family member 3
• Synonyms gene
  • PRNPIP
• Synonyms gene name
  • prion protein interacting protein
• Synonyms
  • FLJ22943
  • PINT1
• Synonyms name
  • enhanced RNAi three prime mRNA exonuclease homolog 3 (C.elegans)
  • exoribonuclease 3
• GenBank acession
  • AF007157
• Locus
  1p34.1
• Discovery year
  2001-12-13
• Entrez gene record
  79033
• RefSeq identity
  • NM_024066
• Classification
  • Exonucleases
• VEGA ID
  OTTHUMG00000007637

Gene info

• Identity
  HGNC:14952
• Gene
  PPP1R14C
• Long gene name
  protein phosphatase 1 regulatory inhibitor subunit 14C
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 14C
• Synonyms
  • CPI17-like
  • NY-BR-81
  • KEPI
• Synonyms name
  • kinase C-enhanced PP1 inhibitor
• GenBank acession
  • AF308297
• Locus
  6q25.1
• Discovery year
  2001-06-29
• Entrez gene record
  81706
• Pubmed identfication
  • 11948623
• RefSeq identity
  • NM_030949
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000015818

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