Human Kallikrein 3 PicoKine ELISA Kit

  • Catalog number
    EK0816
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of Human Kallikrein 3 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
  • Gene tested
    Human Kallikrein 3
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.005 + 0.118 + 0.224 + 0.378 + 0.702 + 1.346 + 1.694 + 2.178
  • ELISA kit detection limits
    312pg/ml to 20000pg/ml
  • ELISA s sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant human Kallikrein 3
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.8;6.7;12.7;2.6;8.4;14.7 ELISA standard deviations 0.108;0.422;0.686;0.203;0.58;0.853 CV(%);6;6.3;5.4;7.8;6.9;5.8
  • ELISA kit description
    GENTAUR's human Kallikrein 3 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Kallikrein 3 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A18-P261) and your samples are measured with a biotin conjugated polyclonal from goat specific for Kallikrein 3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Kallikrein 3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- Human Kallikrein 3 antibodies;1 ST0000-20;Lyophilized recombinant human Kallikrein 3 standard;20ng/tube×2 AR1107;Biotinylated anti- human Kallikrein 3 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin or EDTA
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human Kallikrein 3 standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    20-25
  • More gene info
    Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3
  • Antigen
    NSO, A18-P261
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Prostate-specific antigen
  • Gene name
    kallikrein-related peptidase 3
  • Alternative
    APS;Gamma seminoprotein;Gamma-seminoprotein;hK3;Kallikrein 3;Kallikrein related peptidase 3;Kallikrein-3;KLK 3;KLK2A1;KLK3;Klk3;KLK3_HUMAN;P 30 antigen;P-30 antigen;P30 antigen;Prostate Specific Antigen;Prostate-specific antigen;Psa;Semenogelase;Seminin
  • Gen protein identity
    P07288
  • Entrez nr
    354
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
  • Gene symbol
    KLK3
  • Short name
    Kallikrein 3 PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Human, Humans
  • Alternative name
    H. sapiens Kallikrein 3 PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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