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Alternative name 1
CD79b, B-cell Antigen Receptor Ig b chain (BCR-Ig beta), 38kD, Clone: SN8, Mab anti-Human; frozen/NO paraffin, IH/flow
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Alternative name 2
CD79b, B-cell Antigen Receptor Ig b chain (BCR-Ig beta), 38kD, Clone: SN8, Mab antibody-Human; frozen/NO paraffin, IH/flow
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Alternative name 3
CD79b, B-cell Antigen Receptor Ig b chain (BCR-Ig beta), 38kD, Clone: SN8, Mab antibody-Homo sapiens; frozen/NO paraffin, IH/flow
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Alternative name 4
anti-CD79b B-cell Antigen Receptor Ig b chain BCR-Ig beta 38kD Clone: SN8
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Alternative name 5
CD79b B-cell Antigen Receptor Ig b chain BCR-Ig beta 38kD Clone: SN8
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
BCRP1, BCRP2, BCRP4, BCR
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Short name
CD79b, B- Antigen Receptor Ig b chain (BCR-Ig beta), 38kD, Clone: SN8, Mouse Monoclonal antibody- ; frozen/NO paraffin, IH/
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Technique
Mouse monoclonal, Antibody, paraffin, antigen, frozen, Mouse, Flow, antibodies against human proteins, antibodies for, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Human, Humans, Mouses
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Alternative name
CD79b molecule, immunoglobulin-associated b, B-cellular protein Receptor Ig b epitope (BCR-Ig b), 38kD, clonality: SN8, Mouse monoclonal (antibody to-)-H. sapiens; -25°C frozen/NO paraffin, IH/flow
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Alternative technique
antibodies, antigenes, murine
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Alternative to gene target
CD79b molecule, immunoglobulin-associated beta, AGM6 and B29 and IGB, CD79B and IDBG-64384 and ENSG00000007312 and 974, protein binding, nuclei, Cd79b and IDBG-213455 and ENSMUSG00000040592 and 15985, CD79B and IDBG-641775 and ENSBTAG00000044204 and 509801
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Tissue
cell, frozen
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