HA-tag (4G3) monoclonal antibody-HRP

• Catalog number
  AP0005MH-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  HA epitope tag; HA Tag; hemagglutinin
• Immunogen
  Synthetic peptide HA-tag.
• Swiss Prot
  N/A
• Reactivity
  All
• Applications
  WB IF IP
• Application_all
  WB:1:1000~1:5000 IF:1:1000 IP:1:100
• Product
  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  HA-tag (4G3) monoclonal antibody-HRP detects over-expressed proteins containing the HA epitope tag.
• Usage
  Western blot (WB) analysis of HA-tag (4G3) mAb-HRP at 1:10000 dillution Lane1:HEK293T whole cell lysate ,untransfected (40μg) Lane2:HEK293T whole cell lysate , transfected with pCMV-HA-IMPDH2(WT) (40μg)
• BiowMW
  N/A
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  HA-tag   4G3   monoclonal  
• Gene symbol
  MYO1G, KRT31, KRT32, KRT33B, KRT35, KRT34, ARHGAP45, KRT33A, AKAP13, PUM3
• Short name
  HA-tag (4G3) monoclonal antibody-
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
• Host
  Mouse
• Label
  tag
• Alternative name
  HA-detection labelled (4G3) monoclonal (antibody to-)-horseradish peroxidase
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:13880
• Gene
  MYO1G
• Long gene name
  myosin IG
• Synonyms
  • HA-2
• Synonyms name
  • minor histocompatibility antigen HA-2
• GenBank acession
  • AF380932
• Locus
  7p13
• Discovery year
  2000-11-28
• Entrez gene record
  64005
• Classification
  • Myosin heavy chains, class I
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000155821

Gene info

• Identity
  HGNC:6448
• Gene
  KRT31
• Long gene name
  keratin 31
• Synonyms gene
  • KRTHA1
• Synonyms gene name
  • keratin, hair, acidic, 1
  • keratin 31, type I
• Synonyms
  • Ha-1
• Synonyms name
  • hard keratin type I 1
• GenBank acession
  • X86570
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3881
• Pubmed identfication
  • 7578244
  • 16831889
• RefSeq identity
  • NM_002277
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133423

Gene info

• Identity
  HGNC:6449
• Gene
  KRT32
• Long gene name
  keratin 32
• Synonyms gene
  • KRTHA2
• Synonyms gene name
  • keratin, hair, acidic, 2
  • keratin 32, type I
• Synonyms
  • Ha-2
• Synonyms name
  • hard keratin type I
• GenBank acession
  • X90761
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3882
• Pubmed identfication
  • 7556444
  • 8823373
  • 16831889
• RefSeq identity
  • NM_002278
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133430

Gene info

• Identity
  HGNC:6451
• Gene
  KRT33B
• Long gene name
  keratin 33B
• Synonyms gene
  • KRTHA3B
• Synonyms gene name
  • keratin, hair, acidic, 3B
  • keratin 33B, type I
• Synonyms
  • Ha-3II
• Synonyms name
  • hard keratin type I 3II
• GenBank acession
  • X82634
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3884
• Pubmed identfication
  • 7565656
  • 16831889
• RefSeq identity
  • NM_002279
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133429

Gene info

• Identity
  HGNC:6453
• Gene
  KRT35
• Long gene name
  keratin 35
• Synonyms gene
  • KRTHA5
• Synonyms gene name
  • keratin, hair, acidic, 5
  • keratin 35, type I
• Synonyms
  • Ha-5
• Synonyms name
  • hard keratin type I 5
• GenBank acession
  • X90762
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3886
• Pubmed identfication
  • 8823373
  • 16831889
• RefSeq identity
  • NM_002280
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133425

Gene info

• Identity
  HGNC:6452
• Gene
  KRT34
• Long gene name
  keratin 34
• Synonyms gene
  • KRTHA4
• Synonyms gene name
  • keratin, hair, acidic, 4
  • keratin 34, type I
• Synonyms
  • Ha-4
• Synonyms name
  • hard keratin type I 4
• GenBank acession
  • Y16790
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3885
• Pubmed identfication
  • 2431943
  • 9756910
  • 16831889
• RefSeq identity
  • NM_021013
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133436

Gene info

• Identity
  HGNC:17102
• Gene
  ARHGAP45
• Long gene name
  Rho GTPase activating protein 45
• Synonyms gene
  • HMHA1
• Synonyms gene name
  • histocompatibility (minor) HA-1
• Synonyms
  • KIAA0223
  • HA-1
• GenBank acession
  • D86976
• Locus
  19p13.3
• Discovery year
  2005-12-06
• Entrez gene record
  23526
• Pubmed identfication
  • 9820596
  • 9039502
• Classification
  • Minor histocompatibility antigens
  • AH/BAR family Rho GTPase activating proteins
  • F-BAR domain containing

Gene info

• Identity
  HGNC:6450
• Gene
  KRT33A
• Long gene name
  keratin 33A
• Synonyms gene
  • KRTHA3A
• Synonyms gene name
  • keratin, hair, acidic, 3A
  • keratin 33A, type I
• Synonyms
  • Ha-3I
  • Krt1-3
• Synonyms name
  • hard keratin type I 3I
• GenBank acession
  • Y16788
• Locus
  17q21.2
• Discovery year
  2004-10-20
• Entrez gene record
  3883
• Pubmed identfication
  • 7565656
  • 16831889
• RefSeq identity
  • NM_004138
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133432

Gene info

• Identity
  HGNC:371
• Gene
  AKAP13
• Long gene name
  A-kinase anchoring protein 13
• Synonyms gene
  • LBC
• Synonyms gene name
  • lymphoid blast crisis oncogene
  • A kinase (PRKA) anchor protein 13
• Synonyms
  • Ht31
  • BRX
  • AKAP-Lbc
  • c-lbc
  • PROTO-LB
  • HA-3
  • ARHGEF13
• GenBank acession
  • M90360
• Locus
  15q25.3
• Discovery year
  1999-09-16
• Entrez gene record
  11214
• Pubmed identfication
  • 9627117
  • 1860836
• RefSeq identity
  • NM_007200
• Classification
  • A-kinase anchoring proteins
  • Dbl family Rho GEFs
  • Minor histocompatibility antigens
  • Pleckstrin homology domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000172205

Gene info

• Identity
  HGNC:29676
• Gene
  PUM3
• Long gene name
  pumilio RNA binding family member 3
• Synonyms gene
  • KIAA0020
• Synonyms gene name
  • KIAA0020
• Synonyms
  • XTP5
  • PEN
  • PUF6
  • hPUF-A
  • HA-8
  • Puf-A
• Synonyms name
  • penguin homolog (Drosophila)
  • minor histocompatibility antigen HA-8
• GenBank acession
  • AL832239
• Locus
  9p24.2
• Discovery year
  2004-01-06
• Entrez gene record
  9933
• Pubmed identfication
  • 7584026
  • 7584028
  • 21266351
• RefSeq identity
  • NM_014878
• Classification
  • Pumilio homology domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000019450

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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