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Synonyms
IL13RA2, IL-13RA2, CD213A2, CD213α2, CT19, CT-19, IL-13R, IL13BP, IL-13BP, IL-13Rα2
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Alternative_names
IL13RA2, IL-13RA2, CD213A2, CD213α2, CT19, CT-19, IL-13R, IL13BP, IL-13BP, IL-13Rα2
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Description
Regulates the effects of both IL13 and IL4.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL13RA2 at 8 ug/ml (100 ul/well) can bind IL13 with a linear range of 0.25 - 10 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL13RA2 at 8 ug/ml (100 ul/well) can bind IL13 with a linear range of 0.25 - 10 ng/ml.
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Molecular Weight
This protein is fused with Fc region of human IgG1 at the C-terminus and has a calculated MW of 63.2 kDa expressed. The predicted N-terminus is Asp27. Protein migrates as 80-90 kDa in reduced SDS-PAGE resulting from glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Interleukin-13 receptor subunit alpha-2, also known as IL13Rα2, IL13Ra2 cluster of differentiation 213A2, CD213A2, CT19, IL-13R, IL13BP, and is a membrane bound protein that in humans is encoded by the IL13RA2 gene. IL13Rα2 is closely related to IL13Rα1, a subunit of the interleukin-13 receptor complex. This protein binds IL13 with high affinity, but lacks any significant cytoplasmic domain, and does not appear to function as a signal mediator. It is, however able to regulate the effects of both IL13 and IL4, despite the fact it is unable to bind directly to the latter. It is also reported to play a role in the internalization of IL13. IL13Rα2 is a component of the cell surface receptors, however, the majority exists in intracellular pools and in soluble form, and thus plays an opposite role as a potent IL13 antagonist compared with IL13Rα1. It also functions as an inhibitor of IL4-dependent pathway probably through the physical interaction between the short intracellular domain of and cytoplasmic domain of IL13Rα2 and the IL4Rα chain. In spite of the failed STAT signaling function, IL13Rα2 dose induce TGF-beta production and fibrosis. Additionally, IL13Rα2has been reported to be abundantly and specifically overexpressed in glioblastoma multiforme.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
IL13RA2
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Short name
CellExp™ IL13RA2/CD213, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ interleukin 13 receptor, a 2/CD213, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
interleukin 13 receptor, alpha 2, CD213A2 and CT19 and IL-13R and IL13BP, IL13RA2 and IDBG-82958 and ENSG00000123496 and 3598, protein binding, Extracellular, Il13ra2 and IDBG-177428 and ENSMUSG00000031289 and 16165, IL13RA2 and IDBG-631325 and ENSBTAG00000020022 and 616008
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