Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ELISA's application For quantitative measurement of human IL7R in cell culture supernatant and serum.
More gene info The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Kit Components 20ng/tube
Uniprot Interleukin-7 receptor subunit alpha
Gen protein identity P16871
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
Immunogen Expression system for standard: NSO; Immunogen sequence: E21-I262
Gene tested Human IL7R
ELISA standard curve Concentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.037 + 0.158 + 0.285 + 0.502 + 0.855 + 1.315 + 1.796 + 2.299
Gene name interleukin 7 receptor
ELISA kit description GENTAUR's human IL7R ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL7R was used as capure antibodies on the ELISA test plate. The internal standards (NSO, E21-I262) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL7R will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL7R in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation 20-25
How the elisa works Sample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human IL7R standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human IL7R standard solution and each sample be measured in duplicate.
Alternative CD 127;CD127;CD127 antigen;CDw127;IL 7R alpha;IL 7R;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;IL7R;IL7RA;IL7RA_HUMAN;IL7Ralpha;ILRA;Interleukin 7 receptor alpha chain;Interleukin 7 receptor;Interleukin 7 receptor isoform H5 6;Interleukin-7 receptor subunit alpha
Entrez nr. 3575
ELISA kit detection limits 312pg/ml to 20000pg/ml
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL7R antibodies;1 ST0000-20;Lyophilized recombinant human IL7R standard;20ng/tube×2 AR1107;Biotinylated anti- human IL7R antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Antigen NSO, E21-I262
Gene Name IL7R
Assay range 312pg/ml-20,000pg/ml
Sample Type cell culture supernates and serum.
Target of this ELISA Natural and recombinant human IL7R
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);2.8;8;14.3;3.4;9.1;16.2 ELISA standard deviations 0.168;0.36;0.815;0.245;0.51;1.07 CV(%);6;4.5;5.7;7.2;5.6;6.6
Detector and capure antibodies a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Short name IL7R/CD127 PicoKine ELISA Kit
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name H. sapiens interleukin 7 receptor/CD127 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target interleukin 7 receptor, CD127 and CDW127 and IL-7R-alpha and IL7RA and ILRA, IL7R and IDBG-16161 and ENSG00000168685 and 3575, protein binding, Extracellular, Il7r and IDBG-129135 and ENSMUSG00000003882 and 16197, IL7R and IDBG-630353 and ENSBTAG00000019975 and 521554, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832