Mouse anti TCF-4
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Catalog numberX1070M
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PricePlease ask
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionTCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.
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Antibody come fromHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein.
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Other descriptionProvided as 0.2µm sterile filtered solution in 0.7 M tris-glycine, pH 7.4, 0.105M NaCl, 30% glycerol with 0.035% sodium azide. Protein A/G Chromatography
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Clone6H5.3
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Antigen antibody binding interactionMouse anti TCF-4 Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman, Mouse
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot, Immunohistochemistry, Immunoprecipitation
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Antibody s suited forDetects TCF-4 by Western blot at 1-4 µg/ml. Detects a 66 kDa band in Cos-1 cells transfected with human TCF-4. Can also be used for immunohistochemistry of 4% paraformaldehyde fixed tissues, immunoprecipitation and supershift assays. Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Barker, N., et al. Resticted high level expression of TCF-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999, 154, 29-35 _x000B__x000B_2. Ishitani, M.L., et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999, 399, 798-802_x000B__x000B_3. Korinek, V., et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nature Genetics 1998, 19, 379-383
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolTCF7L2, TCF4-AS2, TCF4-AS1, TCF7, MIR1302-4, PIRC18, TCF4, HGF
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Short nameMouse anti TCF-4
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG2a
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to TCF-4
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nametranscription factor 7 like 2
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Synonyms gene
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Synonyms gene name
- transcription factor 7-like 2 (T-cell specific, HMG-box)
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- TCF/LEF transcription factor family
- Wnt enhanceosome complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCF4 antisense RNA 2
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Locus
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Discovery year2015-04-09
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCF4 antisense RNA 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2015-04-09
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametranscription factor 7
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Synonyms gene name
- transcription factor 7 (T-cell specific, HMG-box)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-08-18
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Entrez gene record
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RefSeq identity
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Classification
- TCF/LEF transcription factor family
- Wnt enhanceosome complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-4
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 18
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nametranscription factor 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namehepatocyte growth factor
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Synonyms gene
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Synonyms gene name
- deafness, autosomal recessive 39
- hepatocyte growth factor (hepapoietin A; scatter factor)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-06-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
