Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ELISA's application For quantitative measurement of human IL-10 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
More gene info Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
Anti-coagulation heparin, EDTA or citrate
Kit Components 10ng/tube
Gen protein identity P22301
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
Immunogen Expression system for standard: E.coli; Immunogen sequence: S19-N178
Gene tested Human IL-10
ELISA standard curve Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.023 + 0.080 + 0.126 + 0.226 + 0.424 + 0.749 + 1.388 + 2.493
Gene name interleukin 10
ELISA kit description GENTAUR's human IL-10 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-10 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, S19-N178) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-10 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-10 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation 20-25
How the elisa works Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-10 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human IL-10 standard solution and each sample is measured in duplicate.
Alternative CSIF;Cytokine synthesis inhibitory factor;GVHDS;IL 10;IL-10;IL10;IL10_HUMAN;IL10A;Interleukin 10;Interleukin-10;Interleukin10;MGC126450;MGC126451;T cell growth inhibitory factor;TGIF
Entrez nr. 3586
ELISA kit detection limits 7.8pg/ml to 500pg/ml(cellculturesupernatant)3.4pg/ml to 250pg/ml(humanserum,plasma)
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-10 antibodies;1 ST0000-10;Lyophilized recombinant human IL-10 standard;10ng/tubex2 AR1107;Biotinylated anti- human IL-10 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Antigen E.coli, S19-N178
Gene Name IL10
Assay range 7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)
Sample Type cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Target of this ELISA Natural and recombinant human IL-10
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);25;112;280;34;184;312 ELISA standard deviations 1.35;5.38;8.68;2.55;14.54;20.9 CV(%);5.4;4.8;3.1;7.5;7.9;6.7
Detector and capure antibodies a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Short name IL-10 PicoKine ELISA Kit
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name H. sapiens Interleukin-10 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832