Cell Meter Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry* is a kit that provides a sensitive tool to monitor ONOO- level in living cells. It is developed as an excellent fluorescent probe, which can specifically react with intercellular ONOO' to generate a bright green fluorescent product. This kit is optimized for flow cytometry.
Component A: DAX-J2™ PON Green ; Component B: DMSO
To order Cell Meter Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*, please use the Cat. Nr.16317 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*
aat supplies other types of Assays as 1.Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
For cells, cell lines and tissues in culture till half confluency.FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
Meter Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for *
Flow Cytometry, intracellular, Assay, Flow
cellular Meter fluorimetry Intracellular Peroxynitrite test reagent *Optimized to measure Flow Cytometry*