GAPDH Loading Control Antibody [Biotin Conjugate]

• Catalog number
  R34262BTN
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
• Form
  Purified antibody
• Conjugation
  Biotin Conjugate
• Clone
  Polyclonal antibody
• Recognised antigen
  GAPDH Loading Control [Biotin Conjugate]
• Host animal
  Goat (Capra aegagrus hircus)
• Clonality
  Polyclonal (goat origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat, Pig ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ICC, ELISA (peptide)
• Recommended dilutions
  Western blot: 0.3-1ug/ml,Immunohistochemistry (FFPE): suitable,Immunocytochemistry: suitable,ELISA (peptide) LOD: 1:64000
• Notes
  Optimal dilution of the GAPDH loading control antibody should be determined by the researcher.1. Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies. A non-animal protein (NAP) blocker is recommended.
• Intented use
  This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  2597
• Purity
  Antigen affinity
• Description
  Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH
• Immunogen
  Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody.
• Storage
  Aliquot and store the GAPDH loading control antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• Additional description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• French translation
  anticorps

• Gene target
  GAPDH   Loading   Conjugate]  
• Gene symbol
  GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
• Short name
  Anti-GAPDH Loading Control [Biotin Conjugate]
• Technique
  Antibody, Control, antibodies against human proteins, antibodies for, controls
• Isotype
  Goat Ig
• Label
  Biotin
• Alternative name
  Antibodies to GAPDH Loading Control [Biotin Conjugate]
• Alternative technique
  antibodies, controls
• Tissue
  control

Gene info

• Identity
  HGNC:55492
• Gene
  GAPDH-DT
• Long gene name
  GAPDH divergent transcript
• Locus
  12p13.31
• Discovery year
  2021-03-12
• Entrez gene record
  112268089
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4141
• Gene
  GAPDH
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase
• Synonyms gene
  • GAPD
• GenBank acession
  • AF261085
• Locus
  12p13.31
• Discovery year
  2001-06-22
• Entrez gene record
  2597
• Pubmed identfication
  • 3170585
• RefSeq identity
  • NM_002046
• VEGA ID
  OTTHUMG00000137379

Gene info

• Identity
  HGNC:24864
• Gene
  GAPDHS
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Synonyms gene
  • GAPDS
• Synonyms
  • GAPDH-2
  • GAPD2
• GenBank acession
  • AJ005371
• Locus
  19q13.12
• Discovery year
  2004-02-10
• Entrez gene record
  26330
• Pubmed identfication
  • 10714828
• RefSeq identity
  • NM_014364
• VEGA ID
  OTTHUMG00000182298

Gene info

• Identity
  HGNC:13950
• Gene
  GAPDHP15
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase pseudogene 15
• Synonyms gene
  • GAPDP15
• Synonyms
  • GAPDH-Lp
  • b55C20.4
• Locus
  6p11.2
• Discovery year
  2003-11-26
• Entrez gene record
  642317
• VEGA ID
  OTTHUMG00000014927

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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