Goat, Affinity purified, Unconjugated
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Catalog number
AS10 819
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Price
Please ask
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Size
2 mg
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Available ordering format
Liquid
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Immunogen
Purified human IgM
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
ELISA (ELISA)
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Connected products
other secondary antibodies to human immunoglobulins
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Recommended dilutions for use
needs to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human IgM (μ or mu chain)
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Possible reactivity
human IgM (μ or mu chain)
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibody reacts with the human IgM (μ or mu chain) based on immunoelectrophoresis.No reactivity is observed with IgG F(ab)'2 fragment or non-immunoglobulin human serum proteins based on immunoelectrophoresis.
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References
to be added when available
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Scientific context
Goat anti-human IgM (μ or mu chain)is a secondary antibody which binds to human IgM (μ or mu chain) in immunological assays.
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Notes
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Goat, , Unconjugated by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
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Latin name
Capra aegagrus hircus
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Conjugation
not conjugated
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Gene target
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Short name
Goat, , Unconjugated
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Technique
affinity, purified, Goat, goats, purifications
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
Unconjugated
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Species
Goats
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Alternative name
caprine, protein enriched, nonconjugated
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Alternative technique
pure, caprine
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