Anti-gamma tubulin (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-1322R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  gamma tubulin Centrosome Marker
• Specificity
  This antibody reacts specifically with gamma tubulin
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm
• Antigen Source
  KLH conjugated synthetic peptide derived from human Tubulin gamma-1 chain
• Gene ID
  7283
• Swiss Prot
  P23258
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  TUBG; GCP-1; CDCBM4; TUBGCP1; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1; TUBG1
• Also known as
  gamma tubulin Polyclonal Antibody
• Other name
  Anti-gamma tubulin Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  gamma   tubulin  
• Gene symbol
  TUBG1, TUBG2, TUBG1P
• Short name
  Anti-gamma tubulin
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  gamma tubulin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:12417
• Gene
  TUBG1
• Long gene name
  tubulin gamma 1
• Synonyms gene
  • TUBG
• Synonyms gene name
  • tubulin, gamma polypeptide
  • tubulin, gamma 1
• Synonyms
  • TUBGCP1
• GenBank acession
  • BC000619
• Locus
  17q21.31
• Discovery year
  2000-01-20
• Entrez gene record
  7283
• Pubmed identfication
  • 1904010
• RefSeq identity
  • NM_001070
• Classification
  • Tubulins
• VEGA ID
  OTTHUMG00000180277

Gene info

• Identity
  HGNC:12419
• Gene
  TUBG2
• Long gene name
  tubulin gamma 2
• Synonyms gene name
  • tubulin, gamma 2
• GenBank acession
  • AF225971
• Locus
  17q21.2
• Discovery year
  2000-01-20
• Entrez gene record
  27175
• RefSeq identity
  • NM_016437
• Classification
  • Tubulins
• VEGA ID
  OTTHUMG00000180640

Gene info

• Identity
  HGNC:12418
• Gene
  TUBG1P
• Long gene name
  tubulin gamma 1 pseudogene
• Synonyms gene name
  • tubulin, gamma 1 pseudogene
• GenBank acession
  • AC004985
• Locus
  7p13
• Discovery year
  2000-01-20
• Entrez gene record
  442673
• Pubmed identfication
  • 10903841
• RefSeq identity
  • NG_009488
• VEGA ID
  OTTHUMG00000155247

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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