Mouse Anti-Rat SAP102 Monoclonal IgG1, Clone S19-2

  • Catalog number
    SMC-134D-A390
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S19-2
  • Immunogen
    Fusion protein amino acids 1-120 of rat SAP102
  • Antibody s full description
    Mouse Anti-Rat SAP102 Monoclonal IgG1 Antibody, Clone: S19-2: ATTO 390
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DLG3 Antibody, MRX90 Antibody, NEDLG Antibody, XLMR Antibody, Disks large homolog 3 Antibody, PSD-95/SAP90-related protein 1 Antibody, Synapse-associated protein 102 Antibody, SAP-102 Antibody
  • Raised in
    Mouse
  • Antibody s target
    SAP102
  • Primary research fields
    Neuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Organelle Markers
  • Brandname
    None
  • Antibodies applications
    WB, IHC, IP, ICC/IF, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
  • Antibody in cell
    Basolateral Cell Membrane , Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Growth Cone
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
  • Bibliography
    1. Muller B.M., et al. (1996) Neuron 17: 255-265. 2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628. 3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682. 4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.
  • Released date
    6-Jul-2009
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_113827.1
  • Gene number
    58948
  • Protein number
    Q62936
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SAP102 Antibody [S19-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-134) Mouse Anti-SAP102 Antibody [S19-2] used in Western Blot (WB) on Rat brain lysates (SMC-134)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    SAP102   Monoclonal   IgG1   S19-2  
  • Gene symbol
    RPS19, DLG3, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2
  • Short name
    Mouse Anti- SAP102 Monoclonal IgG1, Clone S19-2
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 390
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat SAP102 monoclonal IgG1, clonality S19-2
  • Alternative technique
    murine, antibodies
  • Clone name
    S19-2
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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