Apoptosis-Inducing Factor Antibody (Mitochondrial marker)

• Catalog number
  R31356
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Apoptosis-Inducing Factor (Mitochondrial marker)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Apoptosis-Inducing Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Apoptosis-Inducing Factor antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O95831
• Purity
  Antigen affinity
• Description
  Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
• Immunogen
  An amino acid sequence from the C-terminus of human AIFM1 (EQHEDLNEVAKLFNIHED) was used as the immunogen for this Apoptosis-Inducing Factor antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the Apoptosis-Inducing Factor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  This 1 is suited for programmed cell-death studies. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• French translation
  anticorps

• Gene target
  Factor   Mitochondrial  
• Gene symbol
  MTERF1, MRRFP1, TFAMP1
• Short name
  Anti-Apoptosis-Inducing Factor (Mitochondrial marker)
• Technique
  apoptosis, Antibody, antibodies against human proteins, antibodies for, apoptosis pathway, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Apoptosis-Inducing Factor (Mitochondrial marker)
• Alternative technique
  celldeath, antibodies
• Tissue
  mitochondrial

Gene info

• Identity
  HGNC:21463
• Gene
  MTERF1
• Long gene name
  mitochondrial transcription termination factor 1
• Synonyms gene
  • MTERF
• Synonyms gene name
  • mitochondrial transcription termination factor
• GenBank acession
  • Y09615
• Locus
  7q21.2
• Discovery year
  2005-06-27
• Entrez gene record
  7978
• Pubmed identfication
  • 15899902
  • 14744862
  • 7681833
• RefSeq identity
  • NM_006980
• VEGA ID
  OTTHUMG00000156057

Gene info

• Identity
  HGNC:35238
• Gene
  MRRFP1
• Long gene name
  mitochondrial ribosome recycling factor pseudogene 1
• Synonyms gene
  • MRRFP
• Synonyms gene name
  • mitochondrial ribosome recycling factor pseudogene
• Locus
  Xq25
• Discovery year
  2008-10-27
• Entrez gene record
  286423
• RefSeq identity
  • NG_005178
• VEGA ID
  OTTHUMG00000022326

Gene info

• Identity
  HGNC:11636
• Gene
  TFAMP1
• Long gene name
  transcription factor A, mitochondrial pseudogene 1
• Synonyms gene
  • TCF6L1
• Synonyms gene name
  • transcription factor 6-like 1 (mitochondrial transcription factor 1-like)
• Synonyms
  • MTTF1
• Locus
  7p22.3
• Discovery year
  1991-09-13
• Entrez gene record
  260341
• RefSeq identity
  • NR_001288
• VEGA ID
  OTTHUMG00000151494

MeSH Data

• Name
  Radioallergosorbent Test
• Concept
  Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
• Tree numbers
  • E01.370.225.812.735.830
  • E05.200.812.735.830
  • E05.478.566.380.810
  • E05.478.566.639.810
  • E05.478.594.760.830
  • E05.601.470.380.810
  • E05.601.470.639.810
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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