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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
BCAR1 Tyr249
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Specificity
This antibody reacts specifically with BCAR1 (Tyr249)
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Modification
Phosphorylation
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Modification site
Tyr249
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr249
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Gene ID
9564
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
p130 represents one of several known substrates for v-Crk encoded p47. p130 Cas (for Crk-associated substrate) exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
BCAR1 phospho Y249; BCAR1phospho Y249; p-BCAR1p-Tyr249; Breast cancer anti estrogen resistance 1 protein; BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; P130CAS.
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Also known as
BCAR1 (Tyr249) Polyclonal Antibody
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Other name
Anti-BCAR1 (Tyr249) Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
BCAR1
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Short name
Anti-BCAR1 (Tyr249)
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
BCAR1 (Tyr249) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
breast cancer anti-estrogen resistance 1, CAS and CAS1 and CASS1 and CRKAS and P130Cas, BCAR1 and IDBG-42468 and ENSG00000050820 and 9564, protein kinase binding, nuclei, Bcar1 and IDBG-193577 and ENSMUSG00000031955 and 12927, BCAR1 and IDBG-629067 and ENSBTAG00000012002 and
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