Universal cDNA Reverse Transcribed by Oligo dT Primer: Human Normal Tissues

• Catalog number
  C4234565
• Price
  Please ask
• Size
  100 rxn

• Shipping conditions
  Dry Ice
• Category
  DNA
• SubCategory
  Universal cDNA
• Description
  this 1 cDNA is double stranded complementary DNA derived by BioChain from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Goup
  reverse transcription

• Gene target
  Universal   Transcribed   Oligo   Primer   Normal   Tissues  
• Short name
  Universal cDNA Reverse Transcribed by Oligo dT Primer: Normal Tissues
• Technique
  reverse, cDNA, cDNAs
• Species
  Human, Humans
• Alternative name
  Universal complementary Desoxyribonucleic acid Reverse Transcribed by Oligo dT Primer: H. sapiens healthy Tissues
• Alternative technique
  transctription, pcr-sequence, pcr
• Tissue
  cdna, normal

MeSH Data

• Name
  Reverse Transcriptase Polymerase Chain Reaction
• Concept
  Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
• Tree numbers
  • E05.393.620.500.725
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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