Myosin Phosphatase Antibody

• Catalog number
  R30766
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Myosin Phosphatase
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Myosin Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O14974
• Purity
  Antigen affinity
• Description
  PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.
• Immunogen
  An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Myosin   Phosphatase  
• Gene symbol
  PPP1R12B, MPRIPP1, PPP1R12A
• Short name
  Anti-Myosin Phosphatase
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Myosin Phosphatase
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7619
• Gene
  PPP1R12B
• Long gene name
  protein phosphatase 1 regulatory subunit 12B
• Synonyms gene
  • MYPT2
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 12B
  • protein phosphatase 1, regulatory subunit 12B
• Synonyms
  • MGC87886
  • MGC131980
  • PP1bp55
• Synonyms name
  • myosin phosphatase regulatory subunit
  • myosin phosphatase, target subunit 2
• GenBank acession
  • AB003062
• Locus
  1q32.1
• Discovery year
  1998-07-13
• Entrez gene record
  4660
• Pubmed identfication
  • 9570949
• RefSeq identity
  • NM_032105
• Classification
  • Myosin phosphatase targeting family
  • Protein phosphatase 1 regulatory subunits
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000041393

Gene info

• Identity
  HGNC:44594
• Gene
  MPRIPP1
• Long gene name
  myosin phosphatase Rho interacting protein pseudogene 1
• Locus
  3p21.31
• Discovery year
  2012-11-19
• Entrez gene record
  100421374
• VEGA ID
  OTTHUMG00000156454

Gene info

• Identity
  HGNC:7618
• Gene
  PPP1R12A
• Long gene name
  protein phosphatase 1 regulatory subunit 12A
• Synonyms gene
  • MYPT1
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • protein phosphatase 1, regulatory subunit 12A
• Synonyms
  • MBS
  • M130
• Synonyms name
  • myosin phosphatase-targeting subunit 1
  • myosin binding subunit
• GenBank acession
  • D87930
• Locus
  12q21.2-q21.31
• Discovery year
  1997-12-23
• Entrez gene record
  4659
• Pubmed identfication
  • 9286714
• RefSeq identity
  • NM_002480
• Classification
  • Ankyrin repeat domain containing
  • Myosin phosphatase targeting family
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000170100

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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