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Long name
ELISA test kit for Rat Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA
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Synonym name
Rattus Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA
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General description
This is a ready to use kit for performing ELISA tests for detection of Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA in samples from Rat
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Antigen
Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA
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Species reactivity
Rat
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Sample types
Our Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA ELISA kit has been proven to work and successfully detect Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA in Rat
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Kit contents
ELISA test kit for Rat Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISAAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
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Detection wavelengt
450 nm
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Assay procedure
1-4h
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Summary of the prodecure
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
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Applications
Enzyme Linked Immunosorbent Assay
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Shipping storage shelf life
The Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Rat Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA is 6 months after delivery.
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Tips
Due to transportation and handling small amounts of the liquid components of the Rattus Cyclin-dependent kinase inhibitor 2A, isoform 1 ELISA can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
