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Alternative name 1
CD163, Scavenger Receptor, Cystein Rich (SRCR), Macrophage (late stage inflam), Clone: 5C6-FAT, Mab anti-Human, Biotin
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Alternative name 2
CD163, Scavenger Receptor, Cystein Rich (SRCR), Macrophage (late stage inflam), Clone: 5C6-FAT, Mab antibody-Human, Biotin
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Alternative name 3
CD163, Scavenger Receptor, Cystein Rich (SRCR), Macrophage (late stage inflam), Clone: 5C6-FAT, Mab antibody-Homo sapiens, Biotin
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Alternative name 4
anti-CD163 Scavenger Receptor Cystein Rich SRCR Macrophage late stage inflam Clone: 5C6-FAT
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Alternative name 5
CD163 Scavenger Receptor Cystein Rich SRCR Macrophage late stage inflam Clone: 5C6-FAT
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
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Gene target
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Gene symbol
FAT1, CD163, FAT4, CD36
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Short name
CD163, Scavenger Receptor, Cystein Rich (SRCR), Macrophage (late stage inflam), Clone: 5C6-FAT, Mouse Monoclonal antibody- ,
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Label
Biotin
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Species
Human, Humans, Mouses
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Alternative name
CD163 molecule, Scavenger Receptor, Cystein Rich (SRCR), Macrophage (late stage inflam), clonality: 5C6-FAT, Mouse monoclonal (antibody to-)-H. sapiens, biotinilated
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Alternative technique
antibodies, murine
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Alternative to gene target
CD163 molecule, M130 and MM130, CD163 and IDBG-16292 and ENSG00000177575 and 9332, protein binding, Extracellular, Cd163 and IDBG-186721 and ENSMUSG00000008845 and 93671, CD163 and IDBG-639861 and ENSBTAG00000019669 and 533844
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