Full-length recombinant rat GRP78
Antibody's other name
78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regu_x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0011__x0000__x0002__x0000__x0008__x0000__x0003__x0000__x0017__x0000_ _x0000_0_x0000_ _x0000__x0017__x0000__x000B__x0000__x0017__x0000__x000C__x0000__x0017__x0000__x0011__x0000_
Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
WB, ICC/IF, ELISA
Human, Mouse, Rat
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-211 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
See included datasheet or contact our support service
GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.
To be tested
To be tested
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (SMC-211). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-211) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen . Magnification: 60X. Western Blot analysis of Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (SMC-211). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-211) at 1:1000. Mouse Anti-GRP78 Antibody [3G12-1G11] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-211) Mouse Anti-GRP78 Antibody [3G12-1G11] used in Western Blot (WB) on Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates (SMC-211)
Country of production
Total weight (kg)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Rattus norvegicus
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