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Mouse Anti Rat GRP78 Monoclonal IgG1 Kappa Clone 3G12 1G11

Mouse Anti Rat GRP78 Monoclonal IgG1 Kappa Clone 3G12 1G11 is available 19 times from Stressmark antibodies labs

SMC-211D-A655 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-APC | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 428.31 USD

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SMC-211D-RPE | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 426.09 USD

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SMC-211D-BI | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 424.98 USD

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SMC-211D | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 376.15 USD

Catalog number SMC-211D
Price 376.15 USD
Size 100 µg
Antibody's full description Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa Antibody, Clone: 3G12-1G11: Unconjugated
Net weight (g) 0.1
1. Gene info
Identity 5238
Gene HSPA5
Long gene name heat shock protein family A (Hsp70) member 5
Synonyms gene
  • GRP78
Synonyms gene name
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms
  • BiP
Synonyms name
  • glucose-regulated protein, 78kDa
Locus 9q33.3
Discovery year 1991-07-26
Entrez gene record 3309
Classification
  • Heat shock 70kDa proteins
Havana BLAST/BLAT OTTHUMG00000020672
MeSH Data
Name Blotting, Western
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Mouse#.jpg#Rat#.png

SMC-211D-A633 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-PCP | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 428.31 USD

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SMC-211D-A488 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-A700 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-A565 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-HRP | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 416.10 USD

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SMC-211D-FITC | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 420.54 USD

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SMC-211S | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 12 µg | 55.48 USD

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SMC-211D-A390 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 430.52 USD

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SMC-211D-A594 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-STR | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 427.20 USD

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SMC-211D-P594 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 437.18 USD

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SMC-211D-A680 | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 429.42 USD

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SMC-211D-ALP | Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11 size: 100 µg | 422.76 USD

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Clone 3G12-1G11
Immunogen Full-length recombinant rat GRP78
Antibody's category Monoclonal Antibodies
Antibody's other name 78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regu_x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0000__x0000__x0017__x0000__x0001__x0000__x0011__x0000__x0002__x0000__x0008__x0000__x0003__x0000__x0017__x0000_ _x0000_0_x0000_ _x0000__x0017__x0000__x000B__x0000__x0017__x0000__x000C__x0000__x0017__x0000__x0011__x0000_
Raised in Mouse
Antibody's target GRP78
Primary research fields Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
Brandname None
Antibodies' applications WB, ICC/IF, ELISA
Antibody's reactivity Human, Mouse, Rat
Antibody's dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purity Protein G Purified
Antibody buffer for storage PBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration 1 mg/ml
Antibody's specificity Detects ~78kDa.
Storage recommendations -20ºC
Shipping recommendations Blue Ice or 4ºC
Antibody certificate of analysis 1 µg/ml of SMC-211 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
Tissue specificity See included datasheet or contact our support service
Scientific context GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Bibliography 1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.
Released date 1-May-2014
Tested applications To be tested
Tested reactivity To be tested
NCBI number NP_037215.1
Gene number 25617
Protein number P06761
Antibody's datasheet Contact our support service
Representative figure linkmore | details
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (SMC-211). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-211) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen . Magnification: 60X. Western Blot analysis of Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (SMC-211). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-211) at 1:1000. Mouse Anti-GRP78 Antibody [3G12-1G11] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-211) Mouse Anti-GRP78 Antibody [3G12-1G11] used in Western Blot (WB) on Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates (SMC-211)
Warning information Non-hazardous
Country of production Canada
Total weight (kg) 1.4
Stock availability In Stock
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus,Rattus norvegicus
Gene targetGRP78 Monoclonal IgG1 Kappa 3G12-1G11
Short name Mouse Anti- GRP78 Monoclonal IgG1 Kappa, Clone 3G12-1G11
Technique Mouse, anti
Host mouse
Isotype IgG1
Species Rat
Alternative name Mouse antibody to-Rat GRP78 monoclonal IgG1 Kappa, clonality 3G12-1G11
Alternative technique antibodies
Clone name 3G12-1G11
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  • GRP78
  • Monoclonal
  • IgG1
  • Kappa
  • 3G12-1G11
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